According to the attached passage, why were experiments repeated on the larger enclosures?
Earthworms are observed to come to the surface in response to vibrations. People exploit this behavior to gather earthworms in large quantities to sell as bait. A researcher was puzzled as to why earthworms would behave in a way that puts them at risk. He developed an hypothesis that earthworms were reacting to the presence of moles. Moles are voracious predators of worms. Prior to this, many people thought that the vibrations imitated rainfall and the worms were trying to avoid drowning underground.
To test the hypothesis, several experiments and surveys were performed. People leave stake holes in the ground as part of making the underground vibrations they use to gather the worms. Areas near these stake holes were examined for the presence of mole tunnels. Sound recordings of burrowing moles and the vibrations made by human bait hunters were made.
Preliminary experimental containers in the lab measuring 20x25x19 cm were filled with soil to a depth of 15 cm. 50 worms were placed in each container and allowed to acclimate overnight. The responses of earthworms to simulated rainfall, the introduction of moles to the container, and sound recordings of burrowing moles were observed for one hour. Control observations were performed on enclosures where no stimulus was used. Later, larger outdoor enclosures 1x1.2m were filled with soil to a depth of 15 cm. Three hundred worms were placed in each container and allowed to acclimate. The same experiments were repeated on the larger enclosures.
Results from these surveys and experiments are shown in the accompanying figures.
Retrieved from: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0003472
The results on the smaller enclosures did not support the hypothesis, so they tried again with larger enclosures.
The researchers tried to show that the effect occurred in a more natural setting.
The researchers only had a small supply of worms at first, so they had to use small enclosures.
The researchers ran out of ideas of other experiments to try.
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